【Abstract】 Objective To study the anti-influenza virus effects of volatile oil of Lonicerae Japonicae Flos. Methods Different concentrations of Lonicerae Japonicae Flos volatile oil were injected into the allantoic cavity of chicken embryos, and allantoic fluid samples were collected for haemagglutination test. Based on the results of haemagglutination inhibition titration in different concentration groups, the inhibitory efficacy of the volatile oil of Lonicerae Japonicae Flos against influenza virus in chicken embryos was evaluated. A mouse lung adapted strain A/PR/8/34(H1N1) of influenza A virus was dropped into the noses of mice with mildly anesthetised to establish a mouse model of influenza pneumonia. Different concentrations of volatile oil of Lonicerae Japonicae Flos were given prophylactic administration to study its anti-influenza virus effect by lung index. Results The volatile oil of Lonicerae Japonicae Flos could significantly reduce the hemagglutination titer in allantoic fluid of chicken embryos infected with influenza virus (P ＜ 0.01), and had a significant easing effect on influenza virus pneumonia in mice. Conclusion The volatile oil of Lonicerae Japonicae Flos has an obvious inhibitory effect on the proliferation of influenza A virus, and at the same time can exert an anti-influenza virus effect by alleviating the symptoms of influenza viral pneumonia.