Objective To explore and analyze the effect of bundled prevention and control measures on infection prevention and control of multi-drugresistant Organism. Methods Patients with multi-drugresistant Organism detected in Zunyi Maternal and Child Health Care Hospital from January 2020 to December 2023 were selected as research subjects, the relevant infection data and prevention and control status of multi-drugresistant Organism were summarized, and fluorescent markers were used to monitor the cleanliness of the surface of bed unit objects. Based on the clustered prevention and control measures and management model adopted by the pre-control group, the post-control group actively strengthened and rectified the relevant problems discovered before the control. Compare the changes in relevant indicators before and after control from January 2020 to June 2021, and from June 2021 to December 2023. Results Comparison of the incidence rates of hospital infections with different types of multi-drugresistant Organism was not statistically significant (P > 0.05). After taking certain measures to intervene, the detection rates of CRAB, MRSA and CRPA were reduced compared with those before control, the pass rates of object surfaces, air and medical staff’s hands were improved compared with those before control, and the adherence rates to the key measures of issuing medical orders for isolation, placing isolation signs, specializing in medical supplies and proper disposal of medical waste were improved compared with the previous ones, and the differences were statistically significant (P ＜ 0.05). Conclusion Targeted cluster prevention and control measures can effectively improve the infection situation of multidrugresistant organism. The preventive effect of drugresistant organism on multiple infections.